Peroxide-eliminating oxidoreductases as biosensors of antioxidant components of medicinal plants.

A A Markaryan, V A Dubinskaya, T D Dargaeva

I. M. Setchenov Moscow Medical Academy, Federal Agency for Public Health and Social Development, Moscow. markaryan@mmascience.ru

Bulletin of experimental biology and medicine 2006 Jul

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A simple in vitro test system based on the use of peroxide-eliminating oxidoreductases (glutathione reductase, glutathione peroxidase) is described. The system allows detection of antioxidant properties of aqueous extracts from raw medicinal plants. The system was used for testing water extract of Baikalskii-6 tea. Antioxidant and, presumably, adaptogenic activity of this tea was detected. In addition to activation of glutathione reductase and glutathione peroxidase, the preparation inhibits erythrocyte catalase and therefore should not be used for detection of antioxidant activity in in vitro screening tests.

Citation

A A Markaryan, V A Dubinskaya, T D Dargaeva. Peroxide-eliminating oxidoreductases as biosensors of antioxidant components of medicinal plants. Bulletin of experimental biology and medicine. 2006 Jul;142(1):55-6