Vasorelaxant effect of flavonoids through calmodulin inhibition: Ex vivo, in vitro, and in silico approaches.

Mariana Torres-Piedra, Mario Figueroa, Oswaldo Hernández-Abreu, Maximiliano Ibarra-Barajas, Gabriel Navarrete-Vázquez, Samuel Estrada-Soto

Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos 62209, Mexico.

Bioorganic & medicinal chemistry 2011 Jan 1

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In our search for potential antihypertensive agents, a series of structurally-related flavonoids was screened. Ex vivo and in vitro biological evaluations indicated that compounds 1-7 displayed an important vasorelaxant effect on the endothelium-intact (E(+)) and -denuded (E(-)) aortic rings test. Their in vitro anti-calmodulin (CaM) properties were determined by means of the inhibitory effect on the activation of the calmodulin-sensitive cAMP phosphodiesterase (PDE1) assay. Molecular modeling experiments were also performed in order to explore the probable binding site of 1-7 with CaM, and the results indicated that they could bind to the protein in the same pocket as trifluoperazine (TFP), a well-known CaM inhibitor. Copyright Â© 2010 Elsevier Ltd. All rights reserved.

Citation

Mariana Torres-Piedra, Mario Figueroa, Oswaldo Hernández-Abreu, Maximiliano Ibarra-Barajas, Gabriel Navarrete-Vázquez, Samuel Estrada-Soto. Vasorelaxant effect of flavonoids through calmodulin inhibition: Ex vivo, in vitro, and in silico approaches. Bioorganic & medicinal chemistry. 2011 Jan 1;19(1):542-6