Inhibition of testicular LDH-X from laboratory animals and man by gossypol and its isomers.

I D Morris, C Higgins, S A Matlin

Journal of reproduction and fertility 1986 Jul

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The inhibitory effect of (+)-, (-)-, (+/-)-gossypol and (+/-)-gossypol acetic acid upon testicular cytosolic LDH-X was measured in vitro. Gossypol acetic acid (0-100 mumol/l) inhibited LDH-X prepared from the testes of the mouse greater than rabbit greater than human greater than rat greater than hamster. There was no relationship between inhibition and in-vivo antifertility activity. LDH activity measured in vitro in serum of men and hamsters was unaffected by gossypol. Gossypol and its isomers were non-competitive inhibitors of human and hamster LDH-X with respect to the coenzyme NADH, competitive inhibitors of human LDH-X and noncompetitive-competitive inhibitors of hamster LDH-X with respect to the substrate alpha-ketobutyrate. Co-incubation with human serum albumin or poly-L-lysine but not lysine protected human and hamster LDH-X from gossypol.

Citation

I D Morris, C Higgins, S A Matlin. Inhibition of testicular LDH-X from laboratory animals and man by gossypol and its isomers. Journal of reproduction and fertility. 1986 Jul;77(2):607-12