Bioluminescent assay of femtomole levels of estrone and estradiol.

J C Nicolas, A M Boussioux, A M Boularan, B Descomps, A Crastes de Paulet

Analytical biochemistry 1983 Nov

filter terms:

A rapid and very sensitive enzymatic assay of estrogens was developed using the transhydrogenase reaction of the human estradiol-17 beta dehydrogenase and bioluminescence for the quantification of NADH accumulated during the first reaction. The assay requires two steps: first, the addition of transhydrogenase buffer, and a few minutes later the addition of the bioluminescence reagent. The method can determine 0.1 to 50 pg of estrogens in the assay, in 1 h, with a coefficient of variation of 5%.

Citation

J C Nicolas, A M Boussioux, A M Boularan, B Descomps, A Crastes de Paulet. Bioluminescent assay of femtomole levels of estrone and estradiol. Analytical biochemistry. 1983 Nov;135(1):141-5