Identification of multispecific organic anion transporter 2 expressed predominantly in the liver.

T Sekine, S H Cha, M Tsuda, N Apiwattanakul, N Nakajima, Y Kanai, H Endou

Kyorin University School of Medicine, Department of Pharmacology and Toxicology, Mitaka, Tokyo, Japan.

FEBS letters 1998 Jun 12

filter terms:

In the present study, we demonstrate that NLT (novel liver-specific transport protein) is a multispecific organic anion transporter of the liver. The amino acid sequence of NLT shows 42% identity to that of the renal multispecific organic anion transporter, OAT1. When expressed in Xenopus laevis oocytes, NLT mediated uptake of organic anions, such as salicylate, acetylsalicylate, PGE2, dicarboxylates and p-aminohippurate. [14C]Salicylate uptake via NLT was saturable (Km = 88.8 +/- 23.4 microM) and sodium-independent. Expression of the mRNA of NLT was detected in the liver and kidney (liver >> kidney). We propose that NLT be renamed OAT2.

Citation

T Sekine, S H Cha, M Tsuda, N Apiwattanakul, N Nakajima, Y Kanai, H Endou. Identification of multispecific organic anion transporter 2 expressed predominantly in the liver. FEBS letters. 1998 Jun 12;429(2):179-82