Alternative splicing of cyclooxygenase-1 mRNA in the human iris.

Melloney J Dröge, Adriaan A van Sorge, Nicolaas J van Haeringen, Wim J Quax, Johan Zaagsma

Department of Pharmaceutical Biology, University Centre for Pharmacy, Groningen, The Netherlands. m.j.droge@farm.rug.nl

Ophthalmic research 2003 May-Jun

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In homogenates of the human iris, the nonsteroidal anti-inflammatory drug (NSAID) S(+)flurbiprofen has been reported to inhibit cyclooxygenase-1 (COX-1) 70-fold more potently than in human whole blood. We hypothesized that this difference may be due to alternative splicing of COX-1 mRNA in the human iris or in whole blood. In this study, we have identified a similar COX-1 splice variant (COX-1SV) in both tissues with comparable COX-1/COX-1SV expression ratios. Therefore, we conclude that the difference in IC(50) values of S(+)flurbiprofen towards COX-1 in the human iris and human whole blood is not related to differences in the occurrence of spliced COX-1. Copyright 2003 S. Karger AG, Basel

Citation

Melloney J Dröge, Adriaan A van Sorge, Nicolaas J van Haeringen, Wim J Quax, Johan Zaagsma. Alternative splicing of cyclooxygenase-1 mRNA in the human iris. Ophthalmic research. 2003 May-Jun;35(3):160-3