Molecular recognition of histidine tRNA by histidyl-tRNA synthetase from hyperthermophilic archaeon, Aeropyrum pernix K1.
Yukari Nagatoyo, Jun Iwaki, Satoko Suzuki, Atsushi Kuno, Tsunemi Hasegawa
Graduate School of Science and Technology, Yamagata University, Yamagata 990-8560, Japan.
Nucleic acids symposium series (2004) 2005To investigate the recognition sites of histidine tRNA for histidyl-tRNA synthetase from an extreme hyperthermophilic archaeon, Aeropyrum pernix K1, we examined histidylation activities by using overexpressed histidyl-tRNA synthetase and various histidine tRNA transcripts that were prepared by in vitro transcription system. Results indicated that anticodon was not recognized by the histidyl-tRNA synthetase similar to that of Escherichia coli histidine tRNA recognition system. Discriminator base C73 was weekly recognized and an additional G residue was specifically recognized by the enzyme.
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Yukari Nagatoyo, Jun Iwaki, Satoko Suzuki, Atsushi Kuno, Tsunemi Hasegawa. Molecular recognition of histidine tRNA by histidyl-tRNA synthetase from hyperthermophilic archaeon, Aeropyrum pernix K1. Nucleic acids symposium series (2004). 2005(49):307-8
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PMID: 17150756
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