Correlation Engine 2.0
Clear Search sequence regions

  • 1 do (1)
  • Au (6)
  • carrier proteins (2)
  • cell (7)
  • cell culture (1)
  • electron (2)
  • endocytosis (1)
  • folate receptor (2)
  • folic acid (4)
  • FR (4)
  • glycol (7)
  • gold (2)
  • GPI (2)
  • humans (1)
  • ionic strength (1)
  • particles (2)
  • polymer (1)
  • receptor 2 (1)
  • receptors (4)
  • thioctic acid (4)
  • tumor cells (2)
  • Sizes of these terms reflect their relevance to your search.

    This paper reports the creation of Au nanoparticles (AuNP) that are soluble in aqueous solution over a broad range of pH and ionic strength values and that are capable of selective uptake by folate receptor positive (FR+) cancer cells. A novel poly(ethylene glycol) (PEG) construct with thioctic acid and folic acid coupled on opposite ends of the polymer chain was synthesized for targeting the AuNP to FR+ tumor cells via receptor-mediated endocytosis. These folic acid-PEG-thioctic acid conjugates were grafted onto 10-nm-diameter Au particles in aqueous solution. The resulting folate-PEG-coated nanoparticles do not aggregate over a pH range of from 2 to 12 and at electrolyte concentrations of up to 0.5 M NaCl with particle concentrations as high as 1.5 x 10(13) particles/mL. Transmission electron microscopy was used to document the performance of these coated nanoparticles in cell culture. Selective uptake of folate-PEG grafted AuNPs by KB cells, a FR+ cell line that overexpress the folate receptor, was observed. AuNP uptake was minimal in cells that (1) do not overexpress the folate receptor, (2) were exposed to AuNP lacking the folate-PEG conjugate, or (3) were co-incubated with free folic acid in large excess relative to the folate-PEG grafted AuNP. Understanding this process is an important step in the development of methods that use targeted metal nanoparticles for tumor imaging and ablation.


    Vivechana Dixit, Jeroen Van den Bossche, Debra M Sherman, David H Thompson, Ronald P Andres. Synthesis and grafting of thioctic acid-PEG-folate conjugates onto Au nanoparticles for selective targeting of folate receptor-positive tumor cells. Bioconjugate chemistry. 2006 May-Jun;17(3):603-9

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 16704197

    View Full Text