Crystallization and preliminary crystallographic analysis of winged bean acidic lectin.

N Manoj, V R Srinivas, B Satish, N C Singha, K Suguna

Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India.

Acta crystallographica. Section D, Biological crystallography 1999 Feb

filter terms:

The acidic lectin (WBAII) from the winged bean (Psophocarpus tetragonolobus) binds to the H-antigenic determinant on human erythrocytes and to the T-antigenic disaccharide Gal-beta1,3-GalNAc. Two crystal forms of WBAII were obtained in the presence of methyl-alpha-D-galactose. Form I belongs to space group R3 with unit-cell dimensions a = b = 182.11, c = 44.99 A and has one dimer in the asymmetric unit. Form II belongs to space group C2 with unit-cell dimensions a = 135.36, b = 127.25, c = 139.98 A, beta = 95. 9 degrees and has four dimers in the asymmetric unit. Intensity data were collected to 3.0 A and to 3.5 A from crystals of form I and II, respectively. The structures were solved by the molecular-replacement method using the coordinates of the basic form of winged bean lectin.

Citation

N Manoj, V R Srinivas, B Satish, N C Singha, K Suguna. Crystallization and preliminary crystallographic analysis of winged bean acidic lectin. Acta crystallographica. Section D, Biological crystallography. 1999 Feb;55(Pt 2):564-5