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Upon jasmonate treatment barley leaf segments express a putative ribosome-inactivating protein (JIP60). The influence of this protein on translation in planta has been analysed by using barley plants and tobacco plants transformed with a barley cDNA encoding JIP60. In both plant systems JIP60 exhibited N-glycosidase activity in vivo. The depurination of the 25S rRNA of tobacco and barley ribosomes led to accumulation of translationally inactive polysomes.

Citation

M Dunaeva, C Goebel, C Wasternack, B Parthier, E Goerschen. The jasmonate-induced 60 kDa protein of barley exhibits N-glycosidase activity in vivo. FEBS letters. 1999 Jun 11;452(3):263-6

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PMID: 10386603

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