High-mannose type oligosaccharide-dependent apoptosis in U937 cells induced by pradimicin, a mannose-binding antibiotic.

T Oki, Y Yamazaki, N Nomura, T Furumai, Y Igarashi

Biotechnology Research Center, Toyama Prefectural University, Kosugi, Japan.

The Journal of antibiotics 1999 May

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Cell surface oligosaccharides play a role in a variety of biological events such as cell adhesion and signal transduction. We have shown that BMY-28864, a semi-synthetic analog of pradimicin, induced apoptosis of U937 cells which had been incubated with 1-deoxymannojirimycin, an inhibitor of mannosidase I. BMY-28864 was not cytotoxic to the cells which had been cultivated with other glycosidase inhibitors such as castanospermine and swainsonine. We thus propose that BMY-28864 induces apoptosis by acting on a specific mannose-rich oligosaccharide, presumably (Man)9(GlcNAc)2+.

Citation

T Oki, Y Yamazaki, N Nomura, T Furumai, Y Igarashi. High-mannose type oligosaccharide-dependent apoptosis in U937 cells induced by pradimicin, a mannose-binding antibiotic. The Journal of antibiotics. 1999 May;52(5):449-54