L Bovanová, E Brandsteterová, A Caniová, K Argalásová, A Lux
Department of Analytical Chemistry, Faculty of Chemical Technology, Slovak Technical University, Bratislava, Slovak Republic.
Journal of chromatography. B, Biomedical sciences and applications 1999 Sep 24A chromatographic method was developed for the T-514 determination in Karwinskia leaves, stems and roots. A C18 analytical column and a mobile phase consisting of methanol and McIlvaine buffer (pH 3) were used. T-514 was detected using a diode array detector and the chromatograms were recorded at 269 and 410 nm. A linear dependence of a peak area on the T-514 concentration (r=0.9991) was obtained in the range of 0.126-12.6 microg/ml. Limits of T-514 quantification (signal-to-noise ratio 10) in plant samples were 126 ng/ml at 410 nm and 28 ng/ml at 269 nm. T-514 was extracted from the plant material with ethyl acetate. Optimal extraction conditions were studied: number of extraction steps, volume of extracting agent and extraction time. The extracts were cleaned up using solid-phase extraction (SPE). SPE recoveries of 99.9% and 98.4% were achieved for the T-514 concentrations of 1.4 microg/ml and 0.26 microg/ml, respectively.
L Bovanová, E Brandsteterová, A Caniová, K Argalásová, A Lux. High-performance liquid chromatographic determination of peroxisomicine A1 (T-514) in genus Karwinskia. Journal of chromatography. B, Biomedical sciences and applications. 1999 Sep 24;732(2):405-10
PMID: 10517363
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