Bacterial triterpenoids of the hopane series from the methanotrophic bacteria Methylocaldum spp.: phylogenetic implications and first evidence for an unsaturated aminobacteriohopanepolyol.

J H Cvejic, L Bodrossy, K L Kovács, M Rohmer

Université Louis Pasteur/CNRS, Institut Le Bel, 4 rue Blaise Pascal, 67070, Strasbourg, France.

FEMS microbiology letters 2000 Jan 15

filter terms:

The hopanoid content of the two methanotrophic bacteria Methylocaldum szegediense and Methylocaldum tepidum was investigated. 35-Aminobacteriohopane-30R,31R,32R,33S, 34S-pentol and its 3beta-methyl homologue were present in both strains. In M. tepidum, they were accompanied by 35-aminobacteriohopane-31R,32R,33S, 34S-tetrol and its 3beta-methyl homologue. The side chain structure was identical to those previously reported from two other obligate methanotrophs, Methylococcus capsulatus and Methylomonas methanica. The two Methylocaldum species shared with the Methylococcus species the presence of 3beta-methylhopanoid as well as of a hopanoid releasing adiantol upon H(5)IO(6)/NaBH(4) treatment. A rare feature was in addition found in M. szegediense. The saturated hopanoids were accompanied by an unsaturated aminobacteriohopanepentol with a Delta(11) double bond. Comparison of the hopanoid fingerprints was in accordance with the close phylogenetic relationship of Methylococcus and Methylocaldum. The major difference was the absence of sterols in Methylocaldum which were always detected in the Methylococcus species.

Citation

J H Cvejic, L Bodrossy, K L Kovács, M Rohmer. Bacterial triterpenoids of the hopane series from the methanotrophic bacteria Methylocaldum spp.: phylogenetic implications and first evidence for an unsaturated aminobacteriohopanepolyol. FEMS microbiology letters. 2000 Jan 15;182(2):361-5