Identification of two proteins, S14 and UIP1, that interact with UCH37.

T Li, W Duan, H Yang, M K Lee, F Bte Mustafa, B H Lee, T S Teo

Department of Biochemistry, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore, Singapore.

FEBS letters 2001 Jan 19

filter terms:

By the use of the yeast two-hybrid screen we have identified two proteins that interacted with UCH37: S14, which is a subunit of PA700 and a novel protein, UIP1 (UCH37 interacting protein 1). The interaction of UCH37 with S14 or UIP1 was confirmed by in vitro binding assay and in vivo co-immunoprecipitation analysis. The C-terminal extension of UCH37 is essential for interaction with S14 or UIP1 as shown by the yeast two-hybrid assay and the in vitro binding assay. Furthermore, UIP1 blocked the interaction between UCH37 and S14 in vitro.

Citation

T Li, W Duan, H Yang, M K Lee, F Bte Mustafa, B H Lee, T S Teo. Identification of two proteins, S14 and UIP1, that interact with UCH37. FEBS letters. 2001 Jan 19;488(3):201-5