Structure and characterization of the crown gall opines heliopine, vitopine and ridéopine.

W S Chilton, A Petit, M D Chilton, Y Dessaux

Department of Botany, North Carolina State University, Raleigh, NC 27695, USA.

Phytochemistry 2001 Sep

filter terms:

The crown gall opines heliopine from tumors induced by octopine type Agrobacterium tumefaciens strains A6, A136(pTiB6-806), E9, A652 and 1590-1 and vitopine from tumor induced by grapevine strains S4 and T2 are identical to synthetic N2-(1'R-carboxyethyl)-L-glutamine. Tumors produced by strains S4 and T2 do not contain octopine or lysopine, but they do contain heliopine and the new opine ridéopine identified as N-(4'-aminobutyl)-D-glutamic acid. Grapevine strains S4 and T2 grow normally on tumor heliopine or synthetic heliopine and on tumor and synthetic ridéopine as well as on ridéopine lactam as sole carbon source. While octopine strains A6 and A136(pTiB6-806) do not grow on heliopine, mutant colonies do appear after a few weeks. Heliopine catabolism by octopine strains is not induced by octopine.

Citation

W S Chilton, A Petit, M D Chilton, Y Dessaux. Structure and characterization of the crown gall opines heliopine, vitopine and ridéopine. Phytochemistry. 2001 Sep;58(1):137-42