Fragmentation studies on monensin A and B by accurate-mass electrospray tandem mass spectrometry.

Norberto P Lopes, Christian B W Stark, Hui Hong, Paul J Gates, James Staunton

University of Cambridge, Department of Chemistry, Lensfield Road, Cambridge CB2 1EW, UK.

Rapid communications in mass spectrometry : RCM 2002

filter terms:

Monensin A and B were studied by electrospray ionisation tandem mass spectrometry (ESI-MS/MS) and the fragment ions were confirmed by accurate-mass measurements. Analyses were performed on both a quadrupole time-of-flight (QTOF) and a Fourier-transform ion cyclotron resonance (FTICR) mass spectrometer. The analysis revealed that fragment ions were produced by Grob-Wharton fragmentations and pericyclic rearrangements in addition to various simple neutral losses. A study of the protonated and sodiated sodium salt revealed different fragmentation pathways for these species, thus complementary structural information could be gained. A complete fragmentation pathway of monensin A and B protonated sodium salt [(M-H+Na)+H])+) and sodiated sodium salt [(M-H+Na)+Na](+) is proposed. MS(3) analysis confirmed the separate fragmentation pathways. Copyright 2002 John Wiley & Sons, Ltd.

Citation

Norberto P Lopes, Christian B W Stark, Hui Hong, Paul J Gates, James Staunton. Fragmentation studies on monensin A and B by accurate-mass electrospray tandem mass spectrometry. Rapid communications in mass spectrometry : RCM. 2002;16(5):414-20