Efficacy of common laboratory disinfectants on the infectivity of Cryptosporidium parvum oocysts in cell culture.

Susan C Weir, Nicholas J Pokorny, Ramon A Carreno, Jack T Trevors, Hung Lee

Applied and environmental microbiology 2002 May

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Nine liquid disinfectants were tested for their ability to reduce infectivity of Cryptosporidium parvum oocysts in cell culture. A 4-min exposure to 6% hydrogen peroxide and a 13-min exposure to ammonium hydroxide-amended windshield washer fluid reduced infectivity 1,000-fold. Other disinfectants tested (70% ethanol, 37% methanol, 6% sodium hypochlorite, 70% isopropanol, and three commercial disinfectants) did not reduce the infectivity after a 33-min exposure. The results indicate that hydrogen peroxide and windshield washer fluid or ammonium hydroxide disinfectant may be suitable laboratory disinfectants against C. parvum oocysts.

Citation

Susan C Weir, Nicholas J Pokorny, Ramon A Carreno, Jack T Trevors, Hung Lee. Efficacy of common laboratory disinfectants on the infectivity of Cryptosporidium parvum oocysts in cell culture. Applied and environmental microbiology. 2002 May;68(5):2576-9