The barley Jip23b gene.

Frieder Müller-Uri, Verena Cameron-Mills, John Mundy

Faculty of Pharmacy, Institute of Pharmaceutical Biology, University of Halle-Wittenberg, Hoher Weg 8, D-06120 Halle, Germany. fmueller@biologie.uni-erlangen.de

Biochimica et biophysica acta 2002 Jun 7

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The barley gene (Jip23) encoding a 23,000-Da protein of unknown function was isolated and shown to be induced by jasmonate methyl ester (MeJA) in leaves. 5'upstream Jip23 sequence was isolated and fused to the beta-glucuronidase gene (GUS), and this reporter was introduced by particle bombardment into barley leaves. With 1.8 kb of this Jip23 sequence, GUS expression was enhanced about threefold by jasmonate treatment. This indicates that the Jip23 regulation by jasmonate occurs at the level of transcription.