Seong-Soo Joo, Jae-Kwon Chang, Jeong-Hwan Park, Hee-Chul Kang, Do-Ik Lee
Division of Immunology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
Archives of pharmacal research 2002 DecLectin-conjugated praecoxin A is a compound, which is combined Wheat Germ Agglutinin (WGA) Lectin with praecoxin A and also known to have an anti-tumor activity. In our lab, in order to investigate its immune reaction other than the anti-tumor activity ever known, we examined cytokines such as IL-6 and IL-12 through their mRNA expressions, which are generally secreted by macrophage both in vivo and in vitro. To analyze, we used RT-PCR for total RNAs of macrophages. As a result, we obtained that both in vitro and in vivo, lectin-conjugated praecoxin A showed an interesting increase on IL-6 and IL-12 even though it may be little hard to say the conjugated form is absolutely more effective than that of lectin or praecoxin A alone for immune response activities. Those results suggest that the conjugated form may give an additional opportunity in a future therapeutic use over its immuno activation properties.
Seong-Soo Joo, Jae-Kwon Chang, Jeong-Hwan Park, Hee-Chul Kang, Do-Ik Lee. Immunoactivation of lectin-conjugated praecoxin A on IL-6, IL-12 expression. Archives of pharmacal research. 2002 Dec;25(6):954-63
PMID: 12510853
View Full Text