Characterization of mGluR5R, a novel, metabotropic glutamate receptor 5-related gene.

Brian Bates, Yuhong Xie, Noel Taylor, Jeremy Johnson, Leeying Wu, Seung Kwak, Maria Blatcher, Kamalakar Gulukota, Janet E Paulsen

Wyeth Research, Functional Genomics, 35 Cambridge Park Drive, Cambridge, MA 02140, USA. bbates@wyeth.com

Brain research. Molecular brain research 2002 Dec 30

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We report here the isolation of a novel gene termed mGluR5R (mGluR5-related). The N-terminus of mGluR5R is highly similar to the extracellular domain of metabotropic glutamate receptor 5 (mGluR5) whereas the C-terminus bears similarity to the testis-specific gene, RNF18. mGluR5R is expressed in the human CNS in a coordinate fashion with mGluR5. Although the sequence suggests that mGluR5R may be a secreted glutamate binding protein, we found that when expressed in HEK293 cells it was membrane associated and not secreted. Furthermore, mGluR5R was incapable of binding the metabotropic glutamate receptor class I selective agonist, quisqualate. Although mGluR5R could not form disulfide-mediated covalent homodimers, it was able to form a homomeric complex, presumably through noncovalent interactions. mGluR5R also formed noncovalent heteromeric associations with an engineered construct of the extracellular domain of mGluR5 as well as with full-length mGluR5 and mGluR1alpha. The ability of mGluR5R to associate with mGluR1alpha and mGluR5 suggests that it may be a modulator of class I metabotropic glutamate receptor function.

Citation

Brian Bates, Yuhong Xie, Noel Taylor, Jeremy Johnson, Leeying Wu, Seung Kwak, Maria Blatcher, Kamalakar Gulukota, Janet E Paulsen. Characterization of mGluR5R, a novel, metabotropic glutamate receptor 5-related gene. Brain research. Molecular brain research. 2002 Dec 30;109(1-2):18-33