Cloning, expression and alternative splicing of a novel isoform of human TCP11b gene].

Yong-Xin Ma, Si-Zhong Zhang, Qia-Qing Wu, Yan Sun

The Key Laboratory of Biotherapy, Ministry of Education and Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu 610041, China.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica 2003 Feb

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Based on homology analysis and RT-PCR, a novel isoform of human TCP11 gene was isolated. It encodes a 503 amino acid protein that is highly homologous to the mouse 566 amino acid protein Tcp-11. Tcp-11 is important to sperm function because it may be the receptor of fertilization promoting peptide (FPP). Complicated alternative splicing was found to exist between TCP11a and TCP11b genes. The gene has been mapped to human chromosome band 6p21 by fluorescence in situ hybridization. Results of Northern blot and RT-PCR analysis of RNA extracted from human tissues revealed that the gene was expressed in fertile adult testes only, but neither in azoospermic patient testes, fetal testes nor in other human tissues. Our results suggest that TCP11b gene may be important to human sperm function and male fertility.

Citation

Yong-Xin Ma, Si-Zhong Zhang, Qia-Qing Wu, Yan Sun. Cloning, expression and alternative splicing of a novel isoform of human TCP11b gene]. Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica. 2003 Feb;35(2):182-8