Characterization of the region containing the jcpk PKD gene on mouse Chromosome 10.

S J Price, L R Chittenden, L Flaherty, B O'Dell, L M Guay-Woodford, L Stubbs, E C Bryda

Joan C. Edwards School of Medicine, Marshall University, Department of Microbiology, Immunology and Molecular Genetics, Huntington, WV 25704, USA.

Cytogenetic and genome research 2002

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The jcpk gene on mouse Chromosome 10 causes a severe, early onset form of polycystic kidney disease (PKD) when inherited in an autosomal recessive manner. In order to positionally clone this gene, high resolution genetic and radiation hybrid maps were generated along with a detailed physical map of the approximately 500-kb region containing the jcpk gene. Additionally, sixty-nine kidney-specific ESTs were evaluated as candidates for jcpk and subsequently localized throughout the mouse genome by radiation hybrid mapping analysis. Previous studies indicating non-complementation of the jcpk mutation and 67Gso, a new PKD translocation mutant had suggested that 67Gso represents a new allele of jcpk. Fluorescence in situ hybridization (FISH) analysis using key bacterial artificial chromosome clones from the jcpk critical region, refined the 67Gso breakpoint and provided support for the allelism of jcpk and 67Gso. Copyright 2002 S. Karger AG, Basel

Citation

S J Price, L R Chittenden, L Flaherty, B O'Dell, L M Guay-Woodford, L Stubbs, E C Bryda. Characterization of the region containing the jcpk PKD gene on mouse Chromosome 10. Cytogenetic and genome research. 2002;98(1):61-6