A molecular basis for the endo-beta 1,3-glucanase activity of the thaumatin-like proteins from edible fruits.

Laurence Menu-Bouaouiche, Christelle Vriet, Willy J Peumans, Annick Barre, Els J M Van Damme, Pierre Rougé

Institut de Pharmacologie et Biologie Structurale, UMR-CNRS 5089, 205, route de Narbonne, 31077 Toulouse cedex 4, France.

Biochimie 2003 Jan-Feb

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Fruit-specific thaumatin-like proteins were isolated from cherry, apple and banana, and their enzymatic and antifungal activities compared. Both the apple and cherry possess a moderate endo-beta 1,3-glucanase activity but are devoid of antifugal activity. In contrast, the banana thaumatin-like protein inhibits the in vitro hyphal growth of Verticillium albo-atrum but is virtually devoid of endo-beta 1,3-glucanase activity. Both structural and molecular modeling studies showed that all three thaumatin-like proteins possess an extended electronegatively charged cleft at their surface, which is believed to be a prerequisite for endo-beta 1,3-glucanase activity. Docking experiments showed that the positioning of linear (1,3)-beta-D-glucans in the cleft of the apple and cherry proteins allows an interaction with the glutamic acid residues that are responsible for the hydrolytic cleavage of the glucan. Due to a different positioning in the cleft of the banana thaumatin-like protein, the linear beta-glucans cannot properly interact with the catalytic glutamic acid residues and as a result the protein possesses no enzymatic activity. The possible function of the fruit-specific thaumatin-like proteins is discussed in view of the observed biological activities and structural features.

Citation

Laurence Menu-Bouaouiche, Christelle Vriet, Willy J Peumans, Annick Barre, Els J M Van Damme, Pierre Rougé. A molecular basis for the endo-beta 1,3-glucanase activity of the thaumatin-like proteins from edible fruits. Biochimie. 2003 Jan-Feb;85(1-2):123-31