Expression and promoter analysis of mouse mastrin gene.

Mau-Sun Chang, Chiung-Ya Chen, Chang-Jen Huang, Chi-Chen Fan, Jien-Ming Chu, Yuh-Cheng Yang

Department of Medical Research, Mackay Memorial Hospital, 45 Mingshen Road, Tamshui, 251, Taipei, Taiwan.

Biochemical and biophysical research communications 2003 Aug 1

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Human astrin is a newly identified microtubule-associated protein, which is highly expressed in the testis. Silencing of astrin has resulted in growth arrest and apoptotic cell death. In this study, we describe the cloning and genomic structure of mastrin, the mouse counterpart to astrin. The overall mouse mastrin amino-acid sequence is 66% identical to human astrin. Mastrin protein was demonstrated to localize to mitotic spindles during mitosis. Genomic clones containing mastrin gene were isolated; the gene was found to have 24 exons spanning 24kb of genomic DNA. Deletion analysis of 5(')-flanking sequences demonstrated that the first 120bp proximal to the TATA-less promoter region is necessary for minimal transcription of the mouse mastrin gene.

Citation

Mau-Sun Chang, Chiung-Ya Chen, Chang-Jen Huang, Chi-Chen Fan, Jien-Ming Chu, Yuh-Cheng Yang. Expression and promoter analysis of mouse mastrin gene. Biochemical and biophysical research communications. 2003 Aug 1;307(3):491-7