Study on the function of HSD-3.8 gene encoding a testis-specific protein with yeast two-hybrid system].

Wen Lin, Shi-ying Miao, Lin Zhang, Lin-fang Wang

National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae 2002 Dec

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To explore the protein factors that could interact with the testis-specific protein encoded by HSD-3.8 gene (GenBank Accession Number AF311312) related with female fertilization. Yeast two-hybrid system was used to screen the human ovary MATCHMAKER cDNA library with constructed "bait plasmid" containing the 0.7 kb fragment (HSD-0.7) of HSD-3.8. The interaction with the positive fragments using a series of truncated bait plasmids was investigated. One positive gene fragment was obtained, which coded for 144 amino acids of the C-terminus of human G protein beta subunit 1. Truncated bait plasmids couldn't interact with the fish protein fragment in yeast. The protein encoded by HSD-3.8 gene may function through G protein signal transduction pathway and the interaction depends on the integration of the bait protein.

Citation

Wen Lin, Shi-ying Miao, Lin Zhang, Lin-fang Wang. Study on the function of HSD-3.8 gene encoding a testis-specific protein with yeast two-hybrid system]. Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae. 2002 Dec;24(6):582-7