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We selected baculovirus expression vector system to express human NT-4 with biological activity. The hNT-4 mature peptide-coding sequence is amplified by polymerase chain reaction (PCR), ligated to baculovirus expression vector PacGP67B, and expressed in the insert sf9 cell line. The protein molecular weight of the expressed hNT-4 was about 15,000 by SDS-PAGE, and the hNT-4 antibody could react with this protein in the infected supernatant and total cell by western-blot. The activity of hNT-4 determined by PC12 cell line was definite. The results may aid for studying the effect of the hNT-4 on basic medical research and clinical application.

Citation

X L Ma, B R Huang. Expression of neurotrophin-4(NT-4) gene in baculovirus expression vector system]. Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae. 2001 Apr;23(2):141-4

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PMID: 12905890

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