Analysis of differential accumulation of winged bean Kunitz chymotrypsin inhibitor mRNA species by a sequence-specific termination method.

K Umemoto, Y Habu, T Ohno

Department of Agricultural Chemistry, Hokkaido University, Sapporo, Japan.

Plant molecular biology 1992 Dec

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Winged bean Kunitz chymotrypsin inhibitor (WCI) is encoded by a multigene family and accumulation of its mRNA is restricted in mid-maturation stage seeds and tuberous roots. In this paper, we analyzed the accumulation of mRNA derived from each WCI gene using a novel method: sequence-specific termination analysis. The results demonstrated that the accumulation of each WCI mRNA was differentially regulated in winged bean plants.

Citation

K Umemoto, Y Habu, T Ohno. Analysis of differential accumulation of winged bean Kunitz chymotrypsin inhibitor mRNA species by a sequence-specific termination method. Plant molecular biology. 1992 Dec;20(6):1175-8