Susceptibility change to HBV in primary culture of first trimester human fetal hepatocytes].

Bo Tang, Yu-ming Wang, Fang Wang, Jun Liu, Rui Zhang

Department of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology 2004 Jan

filter terms:

By culturing primary early (8 to 12-weeks-old) human fetal hepatocytes with different conditions, to study the status of cell susceptibility to HBV. During primary culture of 10-weeks-old human fetal hepatocytes with serum-free medium adding different differentiation-induced ingredients, to inoculate cell with HBV at certain time. Cell shape, function and markers of HBV infection are measured. 6 days after seeding, markers of mature hepatocytes are observed in cells cultured with 2.5mmol/L phenobarbital sodium, and these cells show susceptibility to HBV. Other ingredients cannot render hepatocytes susceptible to HBV. Phenobarbital sodium induces differentiation and susceptibility to HBV in primary culture of early human fetal hepatocytes.

Citation

Bo Tang, Yu-ming Wang, Fang Wang, Jun Liu, Rui Zhang. Susceptibility change to HBV in primary culture of first trimester human fetal hepatocytes]. Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology. 2004 Jan;12(1):21-4