Identification of a novel thioredoxin-related protein, PC-TRP, which is preferentially expressed in plasma cells.

Jens Wrammert, Eva Källberg, Tomas Leanderson

Section for Immunology, CMB Lund University, Lund, Sweden.

European journal of immunology 2004 Jan

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Using cDNA subtraction we have identified a previously unknown cDNA, termed plasma cell thioredoxin-related protein (PC-TRP), which was overexpressed in plasma cells in vitro. The full-length 2636-bp PC-TRP cDNA contained an open reading frame coding for 417 amino acids. The cDNA sequence was mapped to a region on mouse chromosome 13 and the PC-TRP gene consists of ten exons distributed over a 28-kb region. The PC-TRP was found to contain three atypical thioredoxin domains and showed functional thioredoxin activity in vitro. A more detailed analysis revealed that PC-TRP was expressed at a high level in plasma cells, both at the mRNA and protein level, while present at very low levels in all other cells and tissues examined. Immunohistological analysis revealed that PC-TRP was expressed at high levels in all plasma cells isolated from bone marrow or lamina propria, irrespective of their Ig isotype. In the spleen, however, 60% of the IgG-expressing plasma cells showed only a dull staining at day 14 after immunization, while >90% of the IgG-secreting cells in the spleen expressed PC-TRP at day 21 after immunization. In addition, an IgG-secreting, PC-TRPdull plasma cell population could also be observed after B cell activation in vitro.

Citation

Jens Wrammert, Eva Källberg, Tomas Leanderson. Identification of a novel thioredoxin-related protein, PC-TRP, which is preferentially expressed in plasma cells. European journal of immunology. 2004 Jan;34(1):137-46