Francois Blondeau, Brigitte Ritter, Patrick D Allaire, Sylwia Wasiak, Martine Girard, Natasha K Hussain, Annie Angers, Valerie Legendre-Guillemin, Line Roy, Daniel Boismenu, Robert E Kearney, Alexander W Bell, John J M Bergeron, Peter S McPherson
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, 3801 University Street, Montreal, QC, Canada H3A 2B4.
Proceedings of the National Academy of Sciences of the United States of America 2004 Mar 16Tandem MS has identified 209 proteins of clathrin-coated vesicles (CCVs) isolated from rat brain. An overwhelming abundance of peptides were assigned to the clathrin coat with a 1:1 stoichiometry observed for clathrin heavy and light chains and a 2:1 stoichiometry of clathrin heavy chain with clathrin adaptor protein heterotetramers. Thirty-two proteins representing many of the known components of synaptic vesicles (SVs) were identified, supporting that a main function for brain CCVs is to recapture SVs after exocytosis. A ratio of vesicle-N-ethylmaleimide-sensitive factor attachment protein receptors to target-N-ethylmaleimide-sensitive factor attachment protein receptors, similar to that previously detected on SVs, supports a single-step model for SV sorting during CCV-mediated recycling of SVs. The uncovering of eight previously undescribed proteins, four of which have to date been linked to clathrin-mediated trafficking, further attests to the value of the current organelle-based proteomics strategy.
Francois Blondeau, Brigitte Ritter, Patrick D Allaire, Sylwia Wasiak, Martine Girard, Natasha K Hussain, Annie Angers, Valerie Legendre-Guillemin, Line Roy, Daniel Boismenu, Robert E Kearney, Alexander W Bell, John J M Bergeron, Peter S McPherson. Tandem MS analysis of brain clathrin-coated vesicles reveals their critical involvement in synaptic vesicle recycling. Proceedings of the National Academy of Sciences of the United States of America. 2004 Mar 16;101(11):3833-8
PMID: 15007177
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