Ana María Díaz, María José Abad, Lidia Fernández, Ana Maria Silván, Javier De Santos, Paulina Bermejo
Department of Pharmacology, Faculty of Pharmacy, University Alcala, Alcala de Henares, 28871 Madrid, Spain.
Life sciences 2004 Apr 2Five phenylpropanoid glycosides isolated from Scrophularia scorodonia L. (Scrophulariaceae), namely angoroside A (1), angoroside C (2), angoroside D (3), acteoside (4) and isoacteoside (5), had been evaluated as potential inhibitors of some macrophage functions involved in the inflammatory process. These compounds have been tested in two experimental systems: ionophore-stimulated mouse peritoneal macrophages and human platelets serve as source of COX-1 and 5-LOX, and mouse peritoneal macrophages stimulated with E. coli LPS are the means of testing for COX-2, NO and TNF-alpha activity. None of compounds assayed had a significant effect on LTC(4)-release from calcium ionophore-stimulated mouse peritoneal macrophages. However, the release of PGE(2) by mouse peritoneal macrophages stimulated with calcium ionophore was inhibited by most of these compounds. In the TXB(2)-release assay, acteoside (4), angoroside A (1) and angoroside C (2) showed a significant effect. These five compounds, except angoroside C (2) significantly inhibited LPS-induced PGE(2), NO and TNF-alpha in a concentration-dependent manner. In LPS-stimulated macrophages, the phenylpropanoid glycoside angoroside C (2) only had activity on NO. These results indicate that the pharmacology of these compounds may participate in the anti-inflammatory effect of Scrophularia scorodonia.
Ana María Díaz, María José Abad, Lidia Fernández, Ana Maria Silván, Javier De Santos, Paulina Bermejo. Phenylpropanoid glycosides from Scrophularia scorodonia: in vitro anti-inflammatory activity. Life sciences. 2004 Apr 2;74(20):2515-26
PMID: 15010262
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