Correlation Engine 2.0
Clear Search sequence regions

The light-absorption ratio variation approach (LARVA) has been described and applied to the quantitative detection of ultramicro amounts of Ni by spectrophotometry, which raises notably the detection sensitivity. The complexation between 1, 5-di(2-hydroxy-5-sulfophenyl)-3-cyanoformazan (DSPCF) and Ni(II) at pH 9.11 was investigated and the binary complex was characterized by the spectral correction technique. Results have shown that deltaAr-1 (deltaAr--light-absorption ratio variation) is linear in the range of Ni(II) between 5 and 200 ng/ml. The limit of detection (3sigma) of Ni(II) is only 1.3 ng/ml. The complexation is selective in the presence of fluoride, hexametaphosphate, ethylene diamine tetraacetate and thioglycollic acid. It has been applied to analysis of water quality with satisfactory results.


Hong-Wen Gao, Si-Qing Xia, Hong-Yan Wang, Jian-Fu Zhao. Light-absorption ratio variation approach to determination of nickel (II) in ng/ml level with 1, 5-di(2-hydroxy-5-sulfophenyl)-3-cyanoformazan. Water research. 2004 Mar;38(6):1642-50

Expand section icon Mesh Tags

Expand section icon Substances

PMID: 15016542

View Full Text