A thrombin inhibitor from the ixodid tick, Amblyomma hebraeum.

Ren Lai, Hajime Takeuchi, Jan Jonczy, Huw H Rees, Philip C Turner

Cell Regulation and Signalling Division, School of Biological Sciences, University of Liverpool, Biosciences Building, Crown Street, Liverpool L69 7ZB, UK.

Gene 2004 Nov 24

filter terms:

A novel thrombin inhibitor named Amblin was identified from the haemolymph of the ixodid (hard) tick, Amblyomma hebraeum, and the coding cDNA was isolated from a tick cDNA library. This cDNA codes for a preprotein of 166 amino acids, including a predicted signal peptide composed of 15 amino acids N-terminal to the mature Amblin. The 151-amino-acid mature Amblin contains 14 cysteines and two Kunitz-like domains. It displays high sequence similarity with a tissue factor pathway inhibitor (TFPI), Ixolaris, from the ixodid tick, Ixodes scapularis, which has 10 cysteines, and a thrombin inhibitor, Boophilin, from the ixodid tick, Boophilus microplus, which has 12 cysteines. Recombinant Amblin specifically inhibited thrombin as efficiently as native Amblin did. This is the first report of a thrombin inhibitor from tick haemolymph.

Citation

Ren Lai, Hajime Takeuchi, Jan Jonczy, Huw H Rees, Philip C Turner. A thrombin inhibitor from the ixodid tick, Amblyomma hebraeum. Gene. 2004 Nov 24;342(2):243-9