Sir-mediated repression can occur independently of chromosomal and subnuclear contexts.

Marc R Gartenberg, Frank R Neumann, Thierry Laroche, Marek Blaszczyk, Susan M Gasser

Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA. gartenbe@umdnj.edu

Cell 2004 Dec 29

filter terms:

Epigenetic mechanisms silence the HM mating-type loci in budding yeast. These loci are tightly linked to telomeres, which are also repressed and held together in clusters at the nuclear periphery, much like mammalian heterochromatin. Yeast telomere anchoring can occur in the absence of silent chromatin through the DNA end binding factor Ku. Here we examine whether silent chromatin binds the nuclear periphery independently of telomeres and whether silencing persists in the absence of anchorage. HMR was excised from the chromosome by inducible site-specific recombination and tracked by real-time fluorescence microscopy. Silent rings associate with the nuclear envelope, while nonsilent rings move freely throughout the nucleus. Silent chromatin anchorage requires the action of either Ku or Esc1. In the absence of both proteins, rings move throughout the nucleoplasm yet remain silent. Thus, transcriptional repression can be sustained without perinuclear anchoring.

Citation

Marc R Gartenberg, Frank R Neumann, Thierry Laroche, Marek Blaszczyk, Susan M Gasser. Sir-mediated repression can occur independently of chromosomal and subnuclear contexts. Cell. 2004 Dec 29;119(7):955-67