Absorption of fumonisin B1 and aminopentol on an in vitro model of intestinal epithelium; the role of P-glycoprotein.

I De Angelis, G Friggè, F Raimondi, A Stammati, F Zucco, F Caloni

Department of Environment and Primary Prevention, Toxicity Mechanism Unit, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Rome, Italy. ideangel@iss.it

Toxicon : official journal of the International Society on Toxinology 2005 Mar 1

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The aim of the present paper is to evaluate the absorption of fumonisin B1 and its principal metabolite, aminopentol on a human intestinal model, Caco-2 cells, cultured on semi-permeable inserts, that reproduces the two different intestinal compartments: luminal (apical) and serosal (basolateral) side. Following separate exposure in apical and in basolateral compartments, aminopentol passage through the cell layer (in particular from basolateral to apical direction) was shown, while it was not observed for the parent compound. The different aminopentol distribution between the two compartments of the culture system, and its variation in presence of verapamil or probenecid (P-gp and MRP inhibitors respectively), strongly suggests the involvement of P-glycoprotein in the influx/efflux mechanisms of aminopentol in the intestinal cells, reducing its oral bioavailability.

Citation

I De Angelis, G Friggè, F Raimondi, A Stammati, F Zucco, F Caloni. Absorption of fumonisin B1 and aminopentol on an in vitro model of intestinal epithelium; the role of P-glycoprotein. Toxicon : official journal of the International Society on Toxinology. 2005 Mar 1;45(3):285-91