Polyclonal antibodies against native weak toxin Naja kaouthia discriminate native weak toxins and some other three-fingered toxins against their denaturated forms.

E V Kryukova, D Yu Mordvintsev, S Daya, Yu N Utkin, V I Tsetlin

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, V-437, Miklukho-Maklaya Street, 16/10, 117997 GSP, Moscow, Russia.

Toxicon : official journal of the International Society on Toxinology 2005 Jul

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Polyclonal antibodies obtained by immunization of rabbits with native form of weak toxin (WTX) from cobra Naja kaouthia venom efficiently interacted with WTX and a weak toxin from Naja oxiana venom, but not so with their denaturated forms. These antibodies could also bind with lower affinity other groups of three-fingered toxins: long-chain alpha-neurotoxins, muscarinic toxins and cytotoxins, but practically did not bind short-chain alpha-neurotoxins. The efficiency of toxin-antibody interaction depends on the group (weak toxins, long or short alpha-neurotoxins, cytotoxins etc.) to which the toxin belongs, but not on species of snake from which the toxin originates. There is a correlation between the results obtained and phylogenetic analysis of the three-fingered toxins which revealed that WTX is very close to other weak toxins, relatively close to long alpha-neurotoxins, cytotoxins and muscarinic toxins, but is distant from the short alpha-neurotoxins.

Citation

E V Kryukova, D Yu Mordvintsev, S Daya, Yu N Utkin, V I Tsetlin. Polyclonal antibodies against native weak toxin Naja kaouthia discriminate native weak toxins and some other three-fingered toxins against their denaturated forms. Toxicon : official journal of the International Society on Toxinology. 2005 Jul;46(1):24-30