Kaori Morimoto, Isao Nishimori, Tamotsu Takeuchi, Takuhiro Kohsaki, Nobuto Okamoto, Takahiro Taguchi, Shigeru Yunoki, Ryohei Watanabe, Yuji Ohtsuki, Saburo Onishi
Virchows Archiv : an international journal of pathology 2005 JulThree isoforms of carbonic anhydrase-related protein (CA-RP) are evolutionally well conserved among the CA gene family but lack classical CA activity. Although the biological function of CA-RPs is unknown, overexpression of CA-RP VIII has been reported in certain tumor types. Based on the finding that CA-RPs are commonly expressed in the neuronal cells, we investigated expression of all three CA-RPs in gastrointestinal stromal tumor (GIST). In contrast to no detectable signal of any of the three CA-RPs in intestinal cells of Cajal, immunohistochemical analysis showed distinct cytoplasmic expressions of CA-RPs VIII and XI in 13 (59%) and 20 (91%) of 22 GIST tissue specimens, respectively. The positive signals for both CA-RPs VIII and XI were more intense in the periphery than in the central part of GISTs, whereas no significant signal for CA-RP X expression was observed in any of the GISTs. These expression patterns of CA-RPs were consistently observed by reverse transcription-polymerase chain reaction-Southern blot and immunocytochemistry in the cultured GIST cell line GIST-T1. Ectopic expression of CA-RP XI in GIST-T1 cells induced cell proliferation and invasion in vitro. These findings indicate that CA-RP XI plays a role in the development of GIST.
Kaori Morimoto, Isao Nishimori, Tamotsu Takeuchi, Takuhiro Kohsaki, Nobuto Okamoto, Takahiro Taguchi, Shigeru Yunoki, Ryohei Watanabe, Yuji Ohtsuki, Saburo Onishi. Overexpression of carbonic anhydrase-related protein XI promotes proliferation and invasion of gastrointestinal stromal tumors. Virchows Archiv : an international journal of pathology. 2005 Jul;447(1):66-73
PMID: 15942747
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