Direct interaction between metastasis-associated protein 1 and endophilin 3.

Yuko Aramaki, Katsuhiro Ogawa, Yasushi Toh, Takahiro Ito, Nobuyoshi Akimitsu, Hiroshi Hamamoto, Kazuhisa Sekimizu, Kimihiko Matsusue, Akira Kono, Haruo Iguchi, Soichi Takiguchi

Institute for Clinical Research, National Kyushu Cancer Center, 3-1-1 Notame, Minami-ku, Fukuoka 811-1395, Japan.

FEBS letters 2005 Jul 4

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The yeast two-hybrid system was used to search for partners of mouse metastasis-associated protein 1 (Mta1). Screening of a cDNA library prepared from mouse embryo yielded positive clones coding for endophilin 3. The site of interaction was suggested to be the SH-3-binding domain of Mta1 and SH-3 domain of endophilin 3. This interaction was confirmed by GST pull-down assay in vitro and immunoprecipitation in vivo. The Mta1 and endophilin 3 transcripts were highly expressed in testis and brain. But, Mta1 localized mainly in nucleus and to a lesser extent in cytoplasm while endophilin 3 localized mainly in cytoplasm. If Mta1 functions in cytoplasm, it might be involved in the regulation of endocytosis mediated by endophilin 3.

Citation

Yuko Aramaki, Katsuhiro Ogawa, Yasushi Toh, Takahiro Ito, Nobuyoshi Akimitsu, Hiroshi Hamamoto, Kazuhisa Sekimizu, Kimihiko Matsusue, Akira Kono, Haruo Iguchi, Soichi Takiguchi. Direct interaction between metastasis-associated protein 1 and endophilin 3. FEBS letters. 2005 Jul 4;579(17):3731-6