Correlation Engine 2.0
Clear Search sequence regions


PhoP was previously shown to be important for Yersinia pestis survival in macrophage and under macrophage-induced stresses. In this work, a phoP disruptant of Y. pestis 201 was generated using the Red cloning procedure. The transcription profile of the wild-type Y. pestis was then compared with that of the phoP mutant under Mg2+-limiting conditions. It was revealed that PhoP/PhoQ governed a wide set of cellular pathways in Y. pestis, especially including the positive regulation of many metabolic processes, Mg2+ transport, peptidoglycan remodeling, lipopolysaccharide (LPS) modification and various stress-protective functions. The Mg2+ transport system regulated by PhoP may make Y. pestis to maintain the magnesium homeostasis under low Mg2+ environments. The PhoP-controlled stress-protective functions likely constitute the molecular basis for the observation that mutation of the phoP gene rendered the bacteria more sensitive to various macrophage-induced stresses. Modification of LPS mediated by PhoP is hypothesized to not only neutralize negative charges as normally done by Mg2+ ions, but also mediate the resistance of Y. pestis to antimicrobial peptides. The microarray results provide a population of candidate genes or pathways, and further biochemical experiments are needed to elucidate the PhoP-dependent mechanisms by which Y. pestis survives the antibacterial strategies employed by host macrophages.

Citation

Dongsheng Zhou, Yanping Han, Long Qin, Zeliang Chen, Jingfu Qiu, Yajun Song, Bei Li, Jin Wang, Zhaobiao Guo, Zongmin Du, Xiaoyi Wang, Ruifu Yang. Transcriptome analysis of the Mg2+-responsive PhoP regulator in Yersinia pestis. FEMS microbiology letters. 2005 Sep 1;250(1):85-95

Expand section icon Mesh Tags

Expand section icon Substances


PMID: 16061330

View Full Text