Caveolin-3-anchored microdomains at the rabbit sarcoplasmic reticulum membranes.

Chunlei Li, Wenjuan Duan, Fuyu Yang, Xujia Zhang

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, PR China.

Biochemical and biophysical research communications 2006 Jun 16

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It is generally believed that sphingomyelin- and cholesterol-enriched microdomains can be isolated as detergent-resistant membranes (DRMs) from plasma membrane and organelle membranes. Here, we describe the isolation and characterization of microdomains from sarcoplasmic reticulum (SR) membranes. These SR-derived detergent-resistant membranes (SR-DRMs) enriched in sphingomyelin and cholesterol have a low buoyant density. Immunofluorescence microscopy of SR membranes shows the presence of caveolin-3 in the SR, known as a marker protein of caveolae at plasma membrane. We also demonstrated that significant amount of SERCAs together with caveolin-3 associates with SR-DRMs and are fully functional. Depletion of cholesterol caused the disruption of SR-DRMs.

Citation

Chunlei Li, Wenjuan Duan, Fuyu Yang, Xujia Zhang. Caveolin-3-anchored microdomains at the rabbit sarcoplasmic reticulum membranes. Biochemical and biophysical research communications. 2006 Jun 16;344(4):1135-40