Proteomics-based expression library screening (PELS): a novel method for rapidly defining microbial immunoproteomes.

Indira T Kudva, Bryan Krastins, Haiqing Sheng, Robert W Griffin, David A Sarracino, Phillip I Tarr, Carolyn J Hovde, Stephen B Calderwood, Manohar John

Molecular & cellular proteomics : MCP 2006 Aug

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Current methodologies for global identification of microbial proteins that elicit host humoral immune responses have several limitations and are not ideally suited for use in the postgenomic era. Here we describe a novel application of proteomics, proteomics-based expression library screening, to rapidly define microbial immunoproteomes. Proteomics-based expression library screening is broadly applicable to any cultivable, sequenced pathogen eliciting host antibody responses and hence is ideal for rapidly mining microbial proteomes for targets with diagnostic, prophylactic, and therapeutic potential. In this report, we demonstrate "proof-of-principle" by identifying 207 proteins of the Escherichia coli O157:H7 immunome in bovine reservoirs in only 3 weeks.

Citation

Indira T Kudva, Bryan Krastins, Haiqing Sheng, Robert W Griffin, David A Sarracino, Phillip I Tarr, Carolyn J Hovde, Stephen B Calderwood, Manohar John. Proteomics-based expression library screening (PELS): a novel method for rapidly defining microbial immunoproteomes. Molecular & cellular proteomics : MCP. 2006 Aug;5(8):1514-9