A genetically encoded fluorescent amino acid.
Jiangyun Wang, Jianming Xie, Peter G Schultz
Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Journal of the American Chemical Society 2006 Jul 12The fluorescent amino acid l-(7-hydroxycoumarin-4-yl) ethylglycine 1 has been genetically encoded in E. coli in response to the amber TAG codon. Because of its high fluorescence quantum yield, relatively large Stoke's shift, and sensitivity to both pH and polarity, this amino acid should provide a useful probe of protein localization and trafficking, protein conformation changes, and protein-protein interactions.
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Jiangyun Wang, Jianming Xie, Peter G Schultz. A genetically encoded fluorescent amino acid. Journal of the American Chemical Society. 2006 Jul 12;128(27):8738-9
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PMID: 16819861
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