Molecular characterization of the Tim9 homologue from the methylotrophic yeast Pichia methanolica.

Yoshimi Matsufuji, Tomoyuki Nakagawa, Takashi Ito, Shuki Fujimura, Mika Chikui, Tatsuro Miyaji, Noboru Tomizuka

Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099-2493, Japan.

Yeast (Chichester, England) 2006 Jul 15

filter terms:

In this paper we describe molecular characterization of the TIM9 gene encoding the essential mitochondrial inner-membrane protein in the methylotrophic yeast Pichia methanolica. PmTIM9 contains two exons corresponding to a gene product of 89 amino acid residues and a 140 bp intron. The deduced amino acid sequence exhibited high identity to those of other yeast Tim9ps, and possessed two CX(3)C motifs that contained two cysteine residues conserved among small Tim family proteins. Moreover, PmTIM9 had the ability to partially suppress the temperature sensitivity of Saccharomyces cerevisiae strain tim9-3, suggesting that PmTIM9 is a functional homologue of the ScTIM9 gene.

Citation

Yoshimi Matsufuji, Tomoyuki Nakagawa, Takashi Ito, Shuki Fujimura, Mika Chikui, Tatsuro Miyaji, Noboru Tomizuka. Molecular characterization of the Tim9 homologue from the methylotrophic yeast Pichia methanolica. Yeast (Chichester, England). 2006 Jul 15;23(9):701-6