Diffusion of single star-branched dendrimer-like DNA.

Katherine O Freedman, Janet Lee, Yougen Li, Dan Luo, Victoria B Skobeleva, Pu Chun Ke

Department of Physics and Astronomy, Clemson University, Clemson, South Carolina 29634, USA.

The journal of physical chemistry. B 2005 May 19

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We report a single-molecule fluorescence study on the diffusion of star-branched polymer dendrimer-like DNA (DL-DNA). The DL-DNA molecules were synthesized from ligating Y-shaped DNA to the fourth generation. It was found through single particle tracking that the diffusion coefficient of DL-DNA changes in a nonmonotonic fashion with its increased concentration possibly due to arm arrest and arm retraction. The diffusion of DL-DNA in linear lambda DNA solution displayed a monotonic concentration dependence 1 order of magnitude greater than the diffusion of DL-DNA in DL-DNA solution. This difference is attributed to the different conformation of DL-DNA and lambda DNA and the entanglement of lambda DNA with a large radius of gyration. Our diffusion study facilitates DL-DNA transportation for drug delivery.

Citation

Katherine O Freedman, Janet Lee, Yougen Li, Dan Luo, Victoria B Skobeleva, Pu Chun Ke. Diffusion of single star-branched dendrimer-like DNA. The journal of physical chemistry. B. 2005 May 19;109(19):9839-42