Drosophila Ecp is a novel ribosome associated protein interacting with dRPL5.

Daoyong Wang, Min Xu, Jiabin Liu, Yongqi Wan, Haiteng Deng, Fei Dou, Wei Xie

Department of Genetics and Developmental Biology, Genetics Research Center, Southeast University Medical School, Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Nanjing 210009, Jiangsu Province, China.

Biochimica et biophysica acta 2006 Sep

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Drosophila Ecp is a phylogenetically conserved protein with homologs in most eukaryotes. Studies on Ecp homologs in rat and human suggest those proteins might be involved in cell cycle control, cell proliferation, and learning and memory. However, the molecular function of Ecp itself remains unclear. We show that both the mRNA and protein of ecp are ubiquitously expressed during the entire fly embryogenesis and life cycle. Results of co-immunoprecipitation show that Ecp forms a stable complex with many ribosomal proteins, including dRPL5. The binding of Ecp to dRPL5 was confirmed by GST pulldown. Furthermore, Ecp was found to cosediment with ribosome subunits in a sucrose gradient. These results indicate that Ecp might be a novel ribosome associated protein interacting with dRPL5.

Citation

Daoyong Wang, Min Xu, Jiabin Liu, Yongqi Wan, Haiteng Deng, Fei Dou, Wei Xie. Drosophila Ecp is a novel ribosome associated protein interacting with dRPL5. Biochimica et biophysica acta. 2006 Sep;1760(9):1428-33