Silencing of p29 affects DNA damage responses with UV irradiation.

Po-Chen Chu, Yuh-Cheng Yang, Yen-Ta Lu, Hsiang-Ting Chen, Lung-Chih Yu, Mau-Sun Chang

Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan.

Cancer research 2006 Sep 1

filter terms:

Human p29 is a newly identified nuclear protein whose function is largely undetermined. We found that p29 associated with chromatin, interacted with MCM3, and localized with proliferating cell nuclear antigen foci in the S phase. Silencing of p29 using small interfering RNA duplexes reduced DNA synthesis and increased the expression of p107, a member of the RB family, and of cyclin-dependent kinase inhibitor p21, accompanied with a decreased expression of DNA polymerase alpha. Lethal events consisting of premature chromatin condensation with a reduced Chk1 phosphorylation were observed in p29-depleted cells in response to UV irradiation. Intriguingly, the phosphorylation of ataxia telangectasia-mutated kinases at S1981 was suppressed in p29-depleted HeLa cells with UV irradiation, but not in hydroxyurea- and ionizing radiation-treated cells. Taken together, these results reveal a novel function of p29 in the regulation of DNA replication checkpoint responses.

Citation

Po-Chen Chu, Yuh-Cheng Yang, Yen-Ta Lu, Hsiang-Ting Chen, Lung-Chih Yu, Mau-Sun Chang. Silencing of p29 affects DNA damage responses with UV irradiation. Cancer research. 2006 Sep 1;66(17):8484-91