Resistance of surface-confined telomers with pendent glucosylurea groups against non-specific adsorption of proteins.

A disulfide-carrying random telomer with pendent D-glucosylurea groups (Cys-poly(glucosylureaethyl methacrylate-r-ureaethyl methacrylate), Cys-Poly(GUMA-r-UMA)) was obtained by UV-irradiation of GUMA and UMA in the presence of benzyl N,N-diethyldithiocarbamoyl (BDC) derivative which shows the abilities of initiation, transfer, and termination (iniferter). The disulfide-carrying telomer was accumulated on a gold electrode and colloidal gold-immobilized glass substrate, and the binding of various proteins to the surface of the polymer brush was examined by both cyclic voltammetry (CV) with hydroquinone as a probe, and localized surface plasmon resonance (LSPR) absorption method. The Cys-Poly(GUMA-r-UMA) brush did not show a significant non-specific adsorption of proteins such as bovine serum albumin and egg white lysozyme. Furthermore, sugar binding proteins, concanavalin A (with an affinity for mannose and glucose) and wheat germ agglutinin (with an affinity for N-acetylglucosamine), were only slightly adsorbed to the GUMA-carrying brush, which is in contrast with the prompt and distinct binding of these proteins to the brushes composed of 2-methacryloyloxyethyl D-glucopyranoside and 1-(6'-methacrylamido)hexyl-2-N-acetoamido-2-deoxy-D-glucopyranoside, respectively. The glucosylurea group-carrying telomer brush prepared here might be useful to provide a "bio-inert" surface in bio-medical fields.

Citation

Hiromi Kitano, Makoto Gemmei-Ide, Yasutaka Anraku, Yoshiyuki Saruwatari. Resistance of surface-confined telomers with pendent glucosylurea groups against non-specific adsorption of proteins. Colloids and surfaces. B, Biointerfaces. 2007 Apr 15;56(1-2):188-96

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PMID: 16962744

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