Jun-ichi Nikawa, Masako Yata, Miki Motomura, Nobutaka Miyoshi, Tsuyoshi Ueda, Daisuke Hisada
Department of Bioscience and Bioinformatics, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka, Japan. nikawa@bio.kyutech.ac.jp
Bioscience, biotechnology, and biochemistry 2006 NovYeast Ino2p-Ino4p heterodimeric complex is well known as a transcriptional activator for the genes regulated by inositol and choline, such as the INO1 gene. Apl2p is a large subunit of the yeast adaptin complex, an adaptor complex required for the clathrin coat to bind to the membrane. We found that Ino2p, Ino4p, and Apl2p form a ternary complex. This interaction was initially observed in a yeast two-hybrid study and subsequently verified by co-immunoprecipitation. Ino2p and Ino4p bind to Apl2p in the same region of Apl2p, viz., at the middle part and the C-terminal part. Ino2p and Ino4p bind to Apl2p independently, but more strongly when both are present. Furthermore, a disruption of APL2 together with INO2 or INO4 rendered yeast cells sensitive to oxidative stress. INO2-APL2 double disruptants also showed growth inability in non-fermentable carbon sources, such as glycerol. These results indicate a genetic interaction between APL2, INO2 and INO4 and uncovere novel functions of the Ino2p-Ino4p-Apl2p complex in yeast.
Jun-ichi Nikawa, Masako Yata, Miki Motomura, Nobutaka Miyoshi, Tsuyoshi Ueda, Daisuke Hisada. Ternary complex formation of Ino2p-Ino4p transcription factors and Apl2p adaptin beta subunit in yeast. Bioscience, biotechnology, and biochemistry. 2006 Nov;70(11):2604-12
PMID: 17090927
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