Identification of quantum dot bioconjugates and cellular protein co-localization by hybrid gel blotting.
Department of Biomedical Engineering, Oregon Health & Science University, 3303 SW Bond Avenue, 13B, Portland, Oregon 97239, USA.
Nano letters 2007 AprNew approaches are needed to address the interaction of nanoparticles and cellular proteins at the molecular level. We present a modification of PAGE co-immunoprecipitation, QD-based PA-AGE electrophoresis blotting, and apply this to identify quantum dot (QD) bioconjugate-cellular protein association. This method provides the capability to isolate and evaluate the action of QD bioconjugate-protein complexes in intact cells and to correlate these identified interactions with their location in cells.
Citation
Hong Yan Liu, Tania Q Vu. Identification of quantum dot bioconjugates and cellular protein co-localization by hybrid gel blotting. Nano letters. 2007 Apr;7(4):1044-9
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PMID: 17335273
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