Hepatocellular carcinoma-associated gene 2 interacts with MAD2L2.

Li Li, Yan Shi, Huiling Wu, Bingbing Wan, Pu Li, Li Zhou, Huili Shi, Keke Huo

State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China.

Molecular and cellular biochemistry 2007 Oct

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HCCA2 (hepatocellular carcinoma-associated gene 2) was initially identified as a HCC (hepatocellular carcinoma)-specific protein and subsequently, a long splice variant of HCCA2 was identified as a co-activator of transcription factor YY1 (Yin Yang 1). To investigate the role of HCCA2 in HCC genesis and progression, we screened a human fetal liver cDNA library and identified a novel HCCA2-interacting protein, MAD2L2 (MAD2 mitotic arrest deficient-like 2 (yeast)). The interaction between HCCA2 and MAD2L2 was confirmed by in vitro and in vivo binding assays and the interaction domain was mapped to the N-terminus of HCCA2 by sequential deletion. HCCA2 and MAD2L2 also colocalized in the nucleus of Hela cells. Furthermore, overexpression of HCCA2 led to cell cycle arrest at G0/G1 phase and therefore inhibited cell proliferation. Our research suggests that HCCA2 may play a novel role in cell cycle regulation.

Citation

Li Li, Yan Shi, Huiling Wu, Bingbing Wan, Pu Li, Li Zhou, Huili Shi, Keke Huo. Hepatocellular carcinoma-associated gene 2 interacts with MAD2L2. Molecular and cellular biochemistry. 2007 Oct;304(1-2):297-304